Transient Expression

Transient expression and insertional mutagenesis of Puccinia triticina using biolistics

Genetics / Molecular Biology / Gene expression / Sequence Analysis / Helium / Pressure / Gene Function / Heat Shock / Fungal genome size / Transfection / Base Sequence / Wheat Leaf Rust / BASIDIOMYCOTA / Transient Expression / Molecular Sequence Data / Pressure / Gene Function / Heat Shock / Fungal genome size / Transfection / Base Sequence / Wheat Leaf Rust / BASIDIOMYCOTA / Transient Expression / Molecular Sequence Data

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Functional characterization of a stilbene synthase gene using a transient expression system in planta

Functional Analysis / Plant Biology / Mass Spectrometry / Tobacco / Cell Culture / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression

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Imaging Erg and Jun transcription factor interaction in living cells using fluorescence resonance energy transfer analyses

Fluorescence Microscopy / Transcription Regulation / Confocal Microscopy / Gene expression / Fluorescence Resonance Energy Transfer / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression

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Imaging Erg and Jun transcription factor interaction in living cells using fluorescence resonance energy transfer analyses

Fluorescence Microscopy / Transcription Regulation / Confocal Microscopy / Gene expression / Fluorescence Resonance Energy Transfer / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression

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Functional characterization of a stilbene synthase gene using a transient expression system in planta

Functional Analysis / Plant Biology / Mass Spectrometry / Tobacco / Cell Culture / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression

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pH-sensitivity of YFP provides an intracellular indicator of programmed cell death

Genetics / Plant Biology / Cell Biology / Agricultural Biotechnology / Plant / Cell Death / Programmed cell death / Life Cycle / Onion / Protein Degradation / Epidermal Stem Cells / Transient Expression / Cell Death / Programmed cell death / Life Cycle / Onion / Protein Degradation / Epidermal Stem Cells / Transient Expression

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pH-sensitivity of YFP provides an intracellular indicator of programmed cell death

Genetics / Plant Biology / Cell Biology / Agricultural Biotechnology / Plant / Cell Death / Programmed cell death / Life Cycle / Onion / Protein Degradation / Epidermal Stem Cells / Transient Expression / Cell Death / Programmed cell death / Life Cycle / Onion / Protein Degradation / Epidermal Stem Cells / Transient Expression

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Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025)

Zoology / DNA damage / DNA repair / Phylogeny / Escherichia coli / Animals / Invertebrate Pathology / UV irradiation / Moths / Lymantria Dispar / Amino Acid Sequence / Invertebrate / C-DNA Synthesis / Transient Expression / Animals / Invertebrate Pathology / UV irradiation / Moths / Lymantria Dispar / Amino Acid Sequence / Invertebrate / C-DNA Synthesis / Transient Expression

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Transient expression of ?-glucuronidase following biolistic delivery of foreign DNA into coffee tissues

Plant Biology / Transient Expression

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Prolonged liver-specific transgene expression by a non-primate lentiviral vector

Gene Therapy / Gene expression / Cats / Primates / Metabolic Bone Disease / Humans / Animals / Hepatocellular Carcinoma / Biochemical / Gene Delivery / Human immunodeficiency virus / Transfection / In Vitro Studies / Liver Disease / Transgene Expression / Plasmid DNA / Feline immunodeficiency virus / Biochemistry and cell biology / Transient Expression / Humans / Animals / Hepatocellular Carcinoma / Biochemical / Gene Delivery / Human immunodeficiency virus / Transfection / In Vitro Studies / Liver Disease / Transgene Expression / Plasmid DNA / Feline immunodeficiency virus / Biochemistry and cell biology / Transient Expression

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Transient gene expression in electroporated banana (Musa spp., cv. ?Bluggoe?, ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions

Plant Biology / Gene expression / High Frequency / Polyethylene Glycol / Heat Shock / Gene Transfer / Transient Expression / Gene Transfer / Transient Expression

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Ser72Pro active-site disease mutation in human lysosomal aspartylglucosaminidase: abnormal intracellular processing and evidence for extracellular activation

Biological Sciences / Cercopithecus aethiops / Humans / Animals / Human Molecular Genetics / Endoplasmic Reticulum / Proline / Enzyme / Active site / Enzyme activity / Amino Acid Profile / Lysosomes / Lysosomal Storage Diseases / Transient Expression / Proteolytic activity / Endoplasmic Reticulum / Proline / Enzyme / Active site / Enzyme activity / Amino Acid Profile / Lysosomes / Lysosomal Storage Diseases / Transient Expression / Proteolytic activity

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Molecular and Functional Characterization and Expression Analysis of a Delta6 Fatty Acyl Desaturase cDNA of European Sea Bass (Dicentrarchus labrax L.)

Zoology / Functional Analysis / Olive Oil / Aquaculture / Gene expression / Adipose tissue / Regulation of Gene Expression / Endoplasmic Reticulum / Fish Oil / Fisheries Sciences / Real Time / Enzyme / Marine Fish / Subcellular Localization / Vegetable oil / Dicentrarchus Labrax / Molecular cloning / Sea bass / Fatty Acid / Amino Acid Profile / Open Reading Frame / Enzymatic Activity / Expression analysis / Full Length Movies / Linseed Oil / Transient Expression / Adipose tissue / Regulation of Gene Expression / Endoplasmic Reticulum / Fish Oil / Fisheries Sciences / Real Time / Enzyme / Marine Fish / Subcellular Localization / Vegetable oil / Dicentrarchus Labrax / Molecular cloning / Sea bass / Fatty Acid / Amino Acid Profile / Open Reading Frame / Enzymatic Activity / Expression analysis / Full Length Movies / Linseed Oil / Transient Expression

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Hypoxia-induced human endonuclease G expression suppresses tumor growth in a xenograft model

Oncology / Biotechnology / Cancer / Fluorescence Microscopy / Gene Therapy / Prostate Cancer / Cell line / Humans / Anoxia / Mice / Female / Animals / Male / Cell Death / Cobalt / Cancer Gene Therapy / Time Dependent / Neoplasms / Growth rate / G protein / Cell Proliferation / Tumor Growth / Gene Transfer / Transient Expression / Prostate Cancer / Cell line / Humans / Anoxia / Mice / Female / Animals / Male / Cell Death / Cobalt / Cancer Gene Therapy / Time Dependent / Neoplasms / Growth rate / G protein / Cell Proliferation / Tumor Growth / Gene Transfer / Transient Expression

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Human dehydroepiandrosterone sulfotransferase pharmacogenetics: quantitative Western analysis and gene sequence polymorphisms

Polymorphism / Western blotting / Humans / Liver / Animals / Polymerase Chain Reaction / Enzyme / DNA isolation / Genetic Polymorphism / Dehydroepiandrosterone / Enzyme activity / Amino Acid Profile / Amino Acid Sequence / Recombinant Proteins / Enzymatic Activity / Allele Frequency / Nucleotides / Biochemistry and cell biology / Transient Expression / Polymerase Chain Reaction / Enzyme / DNA isolation / Genetic Polymorphism / Dehydroepiandrosterone / Enzyme activity / Amino Acid Profile / Amino Acid Sequence / Recombinant Proteins / Enzymatic Activity / Allele Frequency / Nucleotides / Biochemistry and cell biology / Transient Expression

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Antiestrogen ICI 164,384 reduces cellular estrogen receptor content by increasing its turnover

Gene expression / Multidisciplinary / Estrogen Receptor / Humans / Mice / Animals / Transfection / DNA binding proteins / Estradiol / Breast Cancer Cells / Down-Regulation / Transient Expression / Animals / Transfection / DNA binding proteins / Estradiol / Breast Cancer Cells / Down-Regulation / Transient Expression

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